THE PHYTOCHEMICAL CONSTITUENTS OF METHANOLIC EXTRACT OF OCIMUM GRATISSIMUM
CHAPTER ONE
1.0 Introduction
1.1 Aim of Study
1.2 Literature Review
1.3 Phytochemicals
1.3.1 Alkaloids
1.3.2 Flavonoids
1.3.2.1 Antioxidant activities
1.3.2.2 Antimicrobial activities
1.3.2.3 Antibacterial activities
1.3.2.3 Antiatherosclerotic effects
1.3.3 Saponins
1.3.4 Tannins
CHAPTER TWO
2.0 Materials and Methods
2.1 Materials Used
2.2 Collection and Identification of samples
2.3 Sample preparation
2.3.1 Drying of the leaves
2.3.2 Pulverisation of the leaves
2.3.3 Extraction Procedure
2.3.4 Filtration Process
2.3.5 Concentration Process
2.4 Phytochemical Analysis
2.4.1 Oualitative Analysis
2.4.1.1 Test for Alkaloids
2.4.1.2 Test for Phytosterol
2.4.1.3 Test for Flavonoids
2.4.1.4 Test for Saponin
2.4.1.5 Test for Phenol and Tannin
2.4.2 Ouantitative Analysis
2.4.2.1 Determination of flavonoid content
2.4.2.2 Determination of tannins content
2.4.2.3 Determination of Saponins content
2.4.2.4 Determination of flavonoid content
2.4.2.5 Determination of Phenolic content
2.4.2.6 Determination of Reducing Power
CHAPTER THREE
3.0 Results
CHAPTER FOUR
4.0 Discussion and Conclusion
4.1 Discussion
4.2 Conclusion
References
LIST OF TABLES
Table 1 – Showing the qualitative analysis results of phytochemical constituents of Ocimum grattissimum
Table 2 – Showing the quantitative analysis of phytochemical constituents of Ocimum grattissimum
Table 3 – Showing the flavonoids determination
Table 4 – Showing the tannin determination
Table 5 – Showing the total phenol determination
Table 6 – Showing the ascorbic acid absorbance
Table 7 – Bioactivity of compounds detected through GC-MS screening of methanolic extract of scent leaves
LIST OF FIGURES
Figxure 1 – Graph showing the flavonoids determination
Figure 2 – Graph showing the tannin determination
Figure 3 – Graph showing the total Phenol determination
Figure 4 – Graph showing the reducing standard curve.
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