THE PHYTOCHEMICAL CONSTITUENTS OF METHANOLIC EXTRACT OF OCIMUM GRATISSIMUM
1.1 Aim of Study
1.2 Literature Review
188.8.131.52 Antioxidant activities
184.108.40.206 Antimicrobial activities
220.127.116.11 Antibacterial activities
18.104.22.168 Antiatherosclerotic effects
2.0 Materials and Methods
2.1 Materials Used
2.2 Collection and Identification of samples
2.3 Sample preparation
2.3.1 Drying of the leaves
2.3.2 Pulverisation of the leaves
2.3.3 Extraction Procedure
2.3.4 Filtration Process
2.3.5 Concentration Process
2.4 Phytochemical Analysis
2.4.1 Oualitative Analysis
22.214.171.124 Test for Alkaloids
126.96.36.199 Test for Phytosterol
188.8.131.52 Test for Flavonoids
184.108.40.206 Test for Saponin
220.127.116.11 Test for Phenol and Tannin
2.4.2 Ouantitative Analysis
18.104.22.168 Determination of flavonoid content
22.214.171.124 Determination of tannins content
126.96.36.199 Determination of Saponins content
188.8.131.52 Determination of flavonoid content
184.108.40.206 Determination of Phenolic content
220.127.116.11 Determination of Reducing Power
4.0 Discussion and Conclusion
LIST OF TABLES
Table 1 – Showing the qualitative analysis results of phytochemical constituents of Ocimum grattissimum
Table 2 – Showing the quantitative analysis of phytochemical constituents of Ocimum grattissimum
Table 3 – Showing the flavonoids determination
Table 4 – Showing the tannin determination
Table 5 – Showing the total phenol determination
Table 6 – Showing the ascorbic acid absorbance
Table 7 – Bioactivity of compounds detected through GC-MS screening of methanolic extract of scent leaves
LIST OF FIGURES
Figxure 1 – Graph showing the flavonoids determination
Figure 2 – Graph showing the tannin determination
Figure 3 – Graph showing the total Phenol determination
Figure 4 – Graph showing the reducing standard curve.
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