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NUTRITIONAL POTENTIAL OF SYNSEPALUM DULCIFICUM PULP AND THE EFFECTOF THE METHANOLIC EXTRACT ON SOME BIOCHEMICAL PARAMETERS IN ALBINO RATS

ABSTRACT

The nutritive and antinutritive compositions of S. dulcificum pulp were analysed to augment the available information on the anti-diabetic effect of the plant. Biochemical parameters like liver function enzymes (ALT, AST, ALP) and bilirubin concentrations,serum total protein, serum albumin and globulin, kidney function parameters (creatinine and urea concentrations), blood glucose, serum lipid profile and lipid peroxidation were determined in rats that were administered different concentrations of the methanolic extract to ascertain their effects. The internal organs (liver and kidney) were also removed and used for histopathological studies. From the result of the study, the proximate composition shows that S. dulcificum contains 7.75% protein, 59.55% moisture content, 4.36% ash, 6.24% crude fibre, 3.26% fat and 18.84% carbohydrate.The result of the mineral analysis shows that S.dulcificum pulp contains 100 mg/g calcium, 24.20 mg/g iron, 9.49 mg/g zinc, 6.22 mg/g copper, 0.01 mg/g chromium and 0.01 mg/g cobalt. Vitamin analyses shows that the S. dulcificum pulp contains 0.04% vitamin A, 22.69% vitamin C, 0.01% vitamin D and 0.02% vitamin K. Antinutrient analyses of the pulp show 5.67% oxalate, 0.03% phytates and 0.02% hemagglutanin. Amino acid profile shows that  S.dulcificum  pulp contains 8.055% tryptophan, 1.35% phenylalanine, 0.7% isoleucine, 0.5% tyrosine, 1.05% methionine, 0.4% proline, 0.69% valine, 1.1% threonine, 0.4% histidine, 0.5% alanine, 1.02% glutamine, 1.6% glutamic acid, 0.7% glycine, 0.3% serine, 1% arginine, 0.1% aspartic acid, 1.23% asparagine, 0.6% lysine and 0.6% leucine. Quantitative phytochemical analysis shows that the pulp contains 3.45% saponins, 57.01%`flavonoids, 7.12% tannins, 0.0001% alkaloids, 0.0001% glycosides, 0.0003% resins, 0.0002% terpenoids, 0.0001% steroids and 0.0003% cyanogenic glycosides.The results of the acute toxicity show that the methanol extract is not toxic to the mice at concentrations up to 5000mg/kg body weight. From the results obtained, the animals receiving 100mg/kg b.w of the methanolic extract showed significantly reduced (p<0.05) serum levels of glucose, bilirubin, low density lipoprotein cholesterol and ALT after the 14 day study compared to the 28 day study. However, no significant difference (p>0.05) was also observed across the groups in their serum ALP, AST, creatinine, urea, cholesterol, TAG, albumin and globulin levels on the 14th day compared with the 28th day. A significant difference (p<0.05) was observed in the  malondaldehyde and serum protein concentrations in the 500mg/kg b.w test group while glucose concentration decreased significantly (p<0.05) in the 100mg/kg b.w and 500mg/kg b.w test group after the 14 day study compared with the 28 day study. High density lipoprotein cholesterol level significantly increased (p<0.05) in the 200mg/kg b.w test group. Histopathological examination shows normal liver architecture across the groups at 100mg/kg b.w, 200mg/kg b.w and 500mg/kg b.w. Kidney sections of rats showing normal glomerulus (G) and renal tubules (arrow) at same concentrations.

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